Vitrification Solution

At present, the main technology for cryopreservation of biological samples is "vitrification" solution, that is, using vitrification cryopreservation medium to make biological samples such as cells enter a "vitrified" state to inhibit the formation of ice crystals during the freezing process. However, achieving "vitrified" state requires the addition of high concentrations (10-15 wt%) of organic solvents, typically dimethyl sulfoxide (DMSO). DMSO has cytotoxicity and epigenetic toxicity, and it is prohibited to be used in non-special and non-irreplaceable situations according to FDA regulations. In addition, it is difficult for the "vitrified" cryopreservation solution to control the "recrystallization" and "ice-growth" caused by "devitrification" during the resuscitation process and result in the damage to cryopreserved objects. For a long time, abandoning DMSO and developing new cryopreservation mediums with non-cytotoxicity, high biocompatibility, and effective control of ice crystal growth has become a difficulty in the international biomedical field, and it is also the future trend.

Bio-Inspired Ice-Controlling Cryopreservation

In order to break through the technical bottleneck of the current "vitrification" cryopreservation of biological samples and eliminate the safety risks caused by the cytotoxicity of DMSO, JCYS is committed to developing the cryopreservative technology system that can control the nucleation and recrystallization/growth of ice crystals.

JCYS's scientific research team has paid attention to the fact that lives in extremely cold areas in nature can control the formation and growth of ice crystals with the help of Antifreeze proteins in their bodies, so as to avoid being damaged by ice crystals. Based on the ice-controlling mechanism of Antifreeze proteins, JCYS proposed the principle of designing the bionic ice-controlling molecules, which completely abandoned the toxic DMSO. A series of DMSO-free bio-inspired cryopreservation medium with capability of extremely miniaturized ice crystal was hence successfully developed for the cryopreservation of variety of biological samples. Under the premise of ensuring high survival rate after cell cryopreservation, our products also significantly improve the quality of cryopreserved cells.