Different types of cells do require different types of frozen fluid. This demand is mainly based on the biological characteristics and functional differences of cells.

First, different types of cells differ in their genetic characteristics, metabolic needs, and sensitivity to the external environment. For example, stem cells have the potential for self-renewal and multidirectional differentiation, so special attention needs to be paid to protecting their genetic stability and differentiation potential when frozen. Immune cells are mainly responsible for recognizing and eliminating foreign pathogens or abnormal cells, and are more sensitive to changes in environmental factors such as temperature, osmotic pressure and pH value. Special attention should be paid to protecting their immune activity and killing ability during cryopreservation. Therefore, for these different types of cells, it is necessary to choose a freeze solution that can maximize the protection of their characteristics and functions.

Second, different types of cells may have different needs for protective agents during freezing. Glycerol and dimethyl sulfoxide (DMSO) are commonly used as protective agents for cell cryopreservation. They can reduce the freezing point, improve the permeability of cell membrane to water, and reduce the damage of ice crystals to cells. However, in some special cases, such as the study of pharmaceutical grade or immune cells, it may be necessary to use freeze-storage solutions that do not contain ingredients of animal origin or have specific additives to meet specific research needs.

In addition, the operational details of different types of cells during cryopreservation and resuscitation may also be different. For example, some cells may need to go through a rigorous procedural cooling process, while others can be stored directly in tanks of liquid nitrogen. Therefore, when selecting the freeze-storage solution, it is also necessary to consider its simplicity and applicability.